Pefachrome® TRY 5274

The line of chromogenic peptide substrates is a range of high quality substrates, which allow to test protease serines.
They target enzymes involved in coagulation and fibrinolysis such as thrombin, Factor Xa, Factor XIIa, kallicrein, activated C protein, plasmin and plasminogen-SK.
These are synthetic peptides that react with proteolytic enzymes by releasing a colour that can be followed by spectrophotometry and whose intensity is proportional to the proteolytic activity of the enzyme.
Typically, such chromogenic substrates are composed of 3 to 5 natural or artificial amino acids. Their structures can be protected in N-terminal to reduce undesirable degradation by aminopeptidases.
Their C-terminal ends are modified so that, during the cleavage of the amide bond, a chromogenic group is released. The most commonly used group is p-nitroaniline (pNA), which absorbs light at a wavelength of 405 nm.