HEMOSTASIS COAGULATION ROUTINE REAGENTS ACTIVATED FACTOR ASSAYS CHROMOGENIC ASSAYS FACTOR IXA
Rox FIX-A
Enzymatic assay kit for human Factor IXa in human Factor IX concentrates.
The Rox Factor IXa kit contains reagents for the colorimetric determination of the activity of FIXa in plasma and in plasma derivatives.
Dilution buffer : additional bottles can be ordered under reference 5-9550, specifying the batch number of the box within the limit of available stocks.
Method / Application
The ROX FIX-A is a chromogenic enzymatic assay kit for the determination of very small amounts of FIXa in human FIX concentrates. The results are expressed in IU. The very low presence of FIXa can be measured. (Specialized hemostasis).
This method is based on the activation of FX by FIXa (considered a contaminant in FIX concentrate) in the presence of FVIII, thrombin, phospholipids and calcium ions.
The amount of FXa generated is determined by hydrolysis of a chromogenic substrate for FXa.
The quantity of para-nitroaniline released during this hydrolysis and measured at 405 nm is proportional to the concentration of FIXa in the reaction medium.
Informations
FIX is a vitamin K dependent glycoprotein synthesized by the liver. FIX can be activated to FIX in FIXa by FXIa or by FVIIa in the presence of phospholipids and calcium.
A person who is deficient in FIX has hemophilia B.
Documentation
Download the product sheetPrice list, safety data sheets and notices are accessible to our registered customers.
Colorimetric assay
References
| 5-950030 | Kit | 2 x 50 tests |
