HEMOSTASIS COAGULATION ROUTINE REAGENTS THROMBOPHILIA PROTEIN C
TECHNOCHROM® Protein C
Quantitative determination of functional protein C in citrated human plasma by chromogenic method in hemostasis.
The TECHNOCHROM® Protein C kit allows functional colorimetric assay of plasma protein C (PC) by amidolytic method, according to a principle of specific activation of PC by a snake venom (Agkistrodon contortrix): PROTAC®. The protein C thus activated hydrolyzes a chromogenic substrate.
The quantity of para-nitroaniline released during this hydrolysis is measured at 405 nm and is proportional to the concentration of protein C present in the reaction medium.
Informations
Protein C (PC) is a vitamin K dependent plasma protein that regulates coagulation by inhibiting FVa and FVIIIa and helps limit the extension of the thrombus.
Numerous clinical studies have shown that a PC deficiency (acquired or congenital) is a risk factor for venous thrombosis. PC is a 62 kDa glycoprotein, synthesized by the liver in the presence of vitamin K.
PC circulates in plasma in an inactive form at a concentration of approximately 4 μg / ml.
Thrombin bound to thrombomodulin loses its procoagulant properties and activates PC into activated PC PCa in the presence of its cofactor, protein S, of calcium and phospholipids, is capable of inactivating activated FV and activated FVIII, true catalysts of coagulation, thus blocking the amplification loop of thrombin generation and limiting extension of the thrombus.
Documentation
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Colorimetric assay
References
| 4-5341013 | Kit | 30 à 60 tests |
