HEMOSTASIS COAGULATION RESEARCH REAGENTS ANSN FLUOROGENIC SUBSTRATES FLUOROGENIC ANSN SUBSTRATES FOR THROMBIN (FIIA)
Fluorogenic substrate ANSN for thrombin
Sequence : Boc-L-FPR-ANSNH-C₂H₅
Formulation : Dimethyl sulfoxide (DMSO)
MW(Da) : 750.9
Km FIIa : 17 µM - Kcat : 58 s-1
Km FXa : 100 µM - Kcat : 0.31 s-1
Km PCa : 40 µM - Kcat : 2.2 s-1
Km t-PA : 47 µM - Kcat : 0.011 s-1
Informations
The kinetic properties identified on the following page will aid in the selection of an appropriate substrate.
The ANSN-based substrates are provided as 10 mM stock solutions in DMSO. Assays are typically conducted in physiologic buffers containing Hepes or Tris, with substrate concentrations ranging from 1 to 100 µM.
The relative change in fluorescence is monitored at a 470 nm emission wavelength with a 352 nm excitation wavelength.
Light artifacts can be minimized by employing a 390 to 450 nm long-pass cutoff filter in the emission beam. The stock substrate solutions in DMSO could remain frozen at 4° C or colder, and should be protected from light. Under these conditions the compounds will remain stable for over one year.
Documentation
Download the product sheetPrice list, safety data sheets and notices are accessible to our registered customers.
References
| 9-SN-20 | Vial | 1 mg |
Related products: 2
MW(Da) : 703.73
Km FIIa : 2 µM – Kcat : 110 s-1
Km FVIIa : 89 µM – Kcat : 0.019 s-1
Km FVIIa/FT : 52 µM – Kcat : 0.76 s-1
Km FXa : 160 µM – Kcat : 3.3 s-1
Km FXIa : 520 µM – Kcat : 92 s-1
Km PCa : 54 µM – Kcat : 72 s-1
Km t-PA : 110 µM – Kcat : 0.71 s-1