Rox Factor IX

 

Human Factor IX enzymatic assay kit.

The Rox Factor IX kit contains reagents for the colorimetric determination of Factor IX activity in plasma and in plasma derivatives.

Dilution buffer : additional bottles can be ordered under reference 5-9050, specifying the batch number of the box within the limit of available stocks.


Method / Application

The ROX FACTOR IX is a chromogenic enzymatic assay kit for the determination of FIX in human FIX concentrates. FIX insufficiency as well as FIX replacement therapy can be monitored. (Specialized hemostasis)

This method is based on the activation of human FIX by human FXIa.
The FIXa thus formed activates FX to FXa in the presence of FVIII, phospholipids and calcium ions.
The amount of FXa generated is determined by hydrolysis of a chromogenic substrate for FXa.
The quantity of para-nitroaniline released during this hydrolysis and measured at 405 nm is proportional to the concentration of FIXa in the reaction medium.


Informations

FIX is a vitamin K dependent glycoprotein synthesized by the liver.
The FIX can be activated in FIX in FIXa by the FXIa or by FVIIa in the presence of phospholipids and calcium.
A person who is deficient in FIX has hemophilia B.


Documentation

Download the product sheet
Price list, safety data sheets and notices are accessible to our registered customers.


Colorimetric assay
 
 
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References

5-900020Kit2 x 50 tests